| Gene | Aard |
| Chromosome | 15 |
| Deletion Start | 52,040,186 |
| Deletion End | 52,045,038 |
| Deletion Size | 4,854 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,040,651 | 52,040,671 | GACCCGATTTCCTCTGGACTC |
| TUP | 52,040,674 | 52,040,698 | TGTTAAGGCCTCCTTGGGTCAAAGC |
| TUR | 52,040,719 | 52,040,739 | GGGCCAATCACTGGACTGTAG |
| TDF | 52,044,932 | 52,044,954 | ATTCCGAGTGTGGAAGCGCATAG |
| TDP | 52,044,955 | 52,044,976 | TGGAGAGTCCGAGGCCACCACT |
| TDR | 52,044,977 | 52,044,995 | GGGCTCAGGGTTGCTTTGG |
PCR Primers
| Primer | Sequence |
| SU | ATCGTCAGCTAGTTCCTCGG |
| SD | TCAGAAGAGGACTGGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13347A-A2 | VGB6 | C57BL/6NTac | |
| 13347A-A7 | VGB6 | C57BL/6NTac | |
| 13347A-C7 | VGB6 | C57BL/6NTac | |
| 13347A-G8 | VGB6 | C57BL/6NTac | |
| 13347A-E10 | VGB6 | C57BL/6NTac | |
| 13347A-E6 | VGB6 | C57BL/6NTac | |
