| Gene | Aasdhppt |
| Chromosome | 9 |
| Deletion Start | 4,309,433 |
| Deletion End | 4,296,623 |
| Deletion Size | 12,811 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,309,137 | 4,309,158 | GACCGTCTTCAGCCTTCGTATC |
| TUP | 4,309,114 | 4,309,136 | CCGGAGGCCTGGGTCACTCACAT |
| TUR | 4,309,081 | 4,309,100 | GTCTCCACAGGCAACCTCAC |
| TDF | 4,297,243 | 4,297,263 | GTCACAGGAAGTCAGGCTCTT |
| TDP | 4,297,211 | 4,297,239 | TGGTTCTCAGTAATTCCTGCATGAGGGTC |
| TDR | 4,297,127 | 4,297,148 | GCAGAAGTGTATCACCAGCAAA |
PCR Primers
| Primer | Sequence |
| SU | GTTGACTCTGAATCTCCGCC |
| SD | GCAGAATAGTTATGAAAGGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13348A-C10 | VGB6 | C57BL/6NTac | |
| 13348A-C8 | VGB6 | C57BL/6NTac | |
