| Gene | Abt1 |
| Chromosome | 13 |
| Deletion Start | 23,423,829 |
| Deletion End | 23,421,969 |
| Deletion Size | 1,861 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,423,620 | 23,423,638 | CAACCTGCTCAGCGCCTAC |
| TUP | 23,423,579 | 23,423,602 | TTTCTTCCAGGCCGAGGGTAAGCC |
| TUR | 23,423,551 | 23,423,571 | CGCATCGTCACCTCTCCTTTC |
| TDF | 23,422,621 | 23,422,643 | CAGGAGGAAAGAAGGGAGCTAAG |
| TDP | 23,422,593 | 23,422,618 | CAGCAAGGACTATACCGAAGGCTGGG |
| TDR | 23,422,571 | 23,422,590 | ACACGCTTGTCTCGGAACTC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGGGTACGCTCTTGATG |
| SD | AGCCTTCCACCACAGTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13352A-B5 | VGB6 | C57BL/6NTac | |
| 13352A-B12 | VGB6 | C57BL/6NTac | |
