| Gene | Adck5 |
| Chromosome | 15 |
| Deletion Start | 76,593,458 |
| Deletion End | 76,595,686 |
| Deletion Size | 2,229 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,594,187 | 76,594,207 | TCGAGAATGAGGGCCGAAATG |
| TUP | 76,594,211 | 76,594,235 | AGCGCTGTGCTCAGGAGCTAAAGCA |
| TUR | 76,594,246 | 76,594,265 | TGCACCCGAGGGATAACAAC |
| TDF | 76,595,120 | 76,595,139 | GGCCCGTGAACACTTTGATG |
| TDP | 76,595,146 | 76,595,170 | TGGAAGTGCTCAAGGCATTGCCACG |
| TDR | 76,595,173 | 76,595,192 | TCGCAGCACTAGCAGCATAG |
PCR Primers
| Primer | Sequence |
| SU | GAGCTGATGGGAGAGACCAG |
| SD | CATCTTGCAGAATGCTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13357A-A1 | VGB6 | C57BL/6NTac | |
| 13357A-H1 | VGB6 | C57BL/6NTac | |
| 13357A-H5 | VGB6 | C57BL/6NTac | |
| 13357A-G6 | VGB6 | C57BL/6NTac | |
| 13357A-F7 | VGB6 | C57BL/6NTac | |
| 13357A-H7 | VGB6 | C57BL/6NTac | |
