| Gene | Adpgk |
| Chromosome | 9 |
| Deletion Start | 59,291,637 |
| Deletion End | 59,315,337 |
| Deletion Size | 23,701 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,292,012 | 59,292,035 | TGCAGGGATAGTCAGCATATTTCC |
| TUP | 59,292,039 | 59,292,059 | CGGTTGGCCCTGCTCCCTGTA |
| TUR | 59,292,079 | 59,292,103 | TGACAGGCTGAGTTGAATTTCTAAG |
| TDF | 59,315,030 | 59,315,052 | AGCCTGTGCCACAGAAACCATAG |
| TDP | 59,315,054 | 59,315,080 | CACCAACCGAGTGTCTCTAAGAGCACC |
| TDR | 59,315,093 | 59,315,115 | AACCGGATTCCAAATGGGATGTC |
PCR Primers
| Primer | Sequence |
| SU | GTACGGATTGGGAACCACAC |
| SD | GGCCAGGTTTCATGAGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13358A-A3 | VGB6 | C57BL/6NTac | |
| 13358B-C11 | VGB6 | C57BL/6NTac | |
| 13358C-H10 | VGB6 | C57BL/6NTac | |
