| Gene | Agpat1 |
| Chromosome | 17 |
| Deletion Start | 34,610,457 |
| Deletion End | 34,612,445 |
| Deletion Size | 1,989 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,611,312 | 34,611,331 | GCCAAGCGTGAGCTACTATG |
| TUP | 34,611,338 | 34,611,357 | CTCTGCTGGGCTGGCCTGCT |
| TUR | 34,611,377 | 34,611,396 | CTGTGCGTTTCCGGTCAATG |
| TDF | 34,611,855 | 34,611,875 | TCACCTCGCCTGGTAAGTTTG |
| TDP | 34,611,877 | 34,611,898 | CAGGATCGACGCCTCTGGGCAG |
| TDR | 34,611,907 | 34,611,928 | CCAGCCTCCTATGAACTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | CCTCTCCATTGGAAGTGAGC |
| SD | TCCTCACTAGGAGACGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13360A-A8 | VGB6 | C57BL/6NTac | |
| 13360A-B7 | VGB6 | C57BL/6NTac | |
| 13360A-C5 | VGB6 | C57BL/6NTac | |
| 13360A-C7 | VGB6 | C57BL/6NTac | |
| 13360A-E8 | VGB6 | C57BL/6NTac | |
| 13360A-B1 | VGB6 | C57BL/6NTac | |
