| Gene | Aldh5a1 |
| Chromosome | 13 |
| Deletion Start | 24,937,565 |
| Deletion End | 24,911,528 |
| Deletion Size | 26,038 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,937,207 | 24,937,225 | CCGGCCTAGAAGGAAGCAG |
| TUP | 24,937,175 | 24,937,203 | TCCACAGAGTGAAACAAAGTGAAACCGGT |
| TUR | 24,937,145 | 24,937,167 | TCTGGCTTTCCGTACTGTGTATC |
| TDF | 24,912,244 | 24,912,265 | CTGAGGTTGGATTGGCAGGTAG |
| TDP | 24,912,222 | 24,912,241 | TTTCTGACTGGCGGCCACCG |
| TDR | 24,912,192 | 24,912,210 | GGAGGCTGGCCTTGACTTG |
PCR Primers
| Primer | Sequence |
| SU | GAACAGAAAGTGCTCCCGTC |
| SD | AAGTTGCACGGTCCCATAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13363A-B2 | VGB6 | C57BL/6NTac | |
| 13363A-B3 | VGB6 | C57BL/6NTac | |
| 13363A-C5 | VGB6 | C57BL/6NTac | |
| 13363A-E7 | VGB6 | C57BL/6NTac | |
| 13363A-G11 | VGB6 | C57BL/6NTac | |
| 13363A-D10 | VGB6 | C57BL/6NTac | |
