| Gene | Ass1 |
| Chromosome | 2 |
| Deletion Start | 31,476,894 |
| Deletion End | 31,501,548 |
| Deletion Size | 24,655 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,477,163 | 31,477,181 | GCTGGCTCTCAGGAAGGTG |
| TUP | 31,477,184 | 31,477,207 | AAAGGATGAAGGCCACCAGGCAGA |
| TUR | 31,477,209 | 31,477,227 | GAGCAGTGGCAAGCTAAGG |
| TDF | 31,501,425 | 31,501,444 | GGGTGGCCTCTGTCCTCAAG |
| TDP | 31,501,446 | 31,501,468 | TTTGTGCCAGCACCTGAGTCCAA |
| TDR | 31,501,509 | 31,501,530 | GCGGTTCTCCACGATGTCAATG |
PCR Primers
| Primer | Sequence |
| SU | CTGACTCCAAGGTCTGCCTC |
| SD | TGACACAAGACAGCCTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13375A-B5 | VGB6 | C57BL/6NTac | |
| 13375A-B7 | VGB6 | C57BL/6NTac | |
| 13375A-C5 | VGB6 | C57BL/6NTac | |
| 13375A-G5 | VGB6 | C57BL/6NTac | |
| 13375A-H3 | VGB6 | C57BL/6NTac | |
