| Gene | Atad2 |
| Chromosome | 15 |
| Deletion Start | 58,134,971 |
| Deletion End | 58,095,464 |
| Deletion Size | 39,508 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,134,357 | 58,134,376 | AGGGACGAACGGCGACATTG |
| TUP | 58,134,326 | 58,134,354 | TGGTTGCACTTTAAGTCTCTTGACAAGCC |
| TUR | 58,134,294 | 58,134,316 | CACCGATAACCACCAACCAGAAG |
| TDF | 58,096,262 | 58,096,284 | AGGTATAGGCAGAAGGATCAGTG |
| TDP | 58,096,232 | 58,096,261 | TCCAAGGTCATCTTCTGCTATGTAGCATGT |
| TDR | 58,096,213 | 58,096,231 | TACCTCAAGCTGGCCTCAA |
PCR Primers
| Primer | Sequence |
| SU | GAACTACAGCTGGAAGAACG |
| SD | TTTGGCAAACAGGAGAAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13376A-D2 | VGB6 | C57BL/6NTac | |
| 13376A-D6 | VGB6 | C57BL/6NTac | |
| 13376A-D7 | VGB6 | C57BL/6NTac | |
| 13376A-E2 | VGB6 | C57BL/6NTac | |
| 13376A-E5 | VGB6 | C57BL/6NTac | |
