| Gene | Prr15l |
| Chromosome | 11 |
| Deletion Start | 96,934,549 |
| Deletion End | 96,934,927 |
| Deletion Size | 379 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,934,662 | 96,934,680 | AGGACCTGACGCAGGAGAC |
| TUP | 96,934,681 | 96,934,705 | CCTCACAGCGACTTTAACAGCCGTC |
| TUR | 96,934,744 | 96,934,764 | GCCTGAGTTGGAGACCTTGAC |
| TDF | 96,934,687 | 96,934,708 | AGCGACTTTAACAGCCGTCTGG |
| TDP | 96,934,723 | 96,934,752 | AAGAACACTAAAGGCAAGCATGTCAAGGTC |
| TDR | 96,934,755 | 96,934,774 | CTTTGAAGCGGCCTGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGCTTGGAAGGAAGGACCTC |
| SD | ACATTGCTCTCCCTGGATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13377A-A4 | VGB6 | C57BL/6NTac | |
| 13377A-D11 | VGB6 | C57BL/6NTac | |
| 13377B-A6 | VGB6 | C57BL/6NTac | |
| 13377B-B12 | VGB6 | C57BL/6NTac | |
| 13377B-C2 | VGB6 | C57BL/6NTac | |
