| Gene | Atf3 |
| Chromosome | 1 |
| Deletion Start | 191,177,467 |
| Deletion End | 191,171,385 |
| Deletion Size | 6,083 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 191,177,309 | 191,177,330 | CGCCATCCAGAATAAACACCTC |
| TUP | 191,177,282 | 191,177,305 | CATCGGATGTCCTCTGCGCTGGAG |
| TUR | 191,177,232 | 191,177,254 | TCAGACTTGGTGACTGACATCTC |
| TDF | 191,171,882 | 191,171,902 | TGCCTTTGGCTCTCAGATTTC |
| TDP | 191,171,855 | 191,171,879 | TTCCATGTCTCCCAACTTCCGAGGC |
| TDR | 191,171,830 | 191,171,852 | GGAAGGTGGTATGAACCCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | ATAAGAAACCTGAAGCCTCAG |
| SD | AGCCACAGTGGAGGATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13378A-A5 | VGB6 | C57BL/6NTac | |
| 13378A-G12 | VGB6 | C57BL/6NTac | |
