| Gene | Atp6v1f |
| Chromosome | 6 |
| Deletion Start | 29,467,820 |
| Deletion End | 29,470,367 |
| Deletion Size | 2,548 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,467,842 | 29,467,861 | CGGTGATTGGAGACGAGGAC |
| TUP | 29,467,868 | 29,467,891 | ACTGGTTTCCTGTTGGGCGGCATA |
| TUR | 29,467,898 | 29,467,918 | AGGGTGGCGGTTCTTGTTTAG |
| TDF | 29,469,929 | 29,469,947 | CACGAGCTGATGTGTGGAC |
| TDP | 29,469,948 | 29,469,970 | TCCAACCCTGAGCTGTGTGGTTC |
| TDR | 29,470,001 | 29,470,021 | GGCCTTCAGCCTTCTCTTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGTCTCGGTCTCTAATAACAC |
| SD | CCTAGGTGAAGCAGCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13382A-A4 | VGB6 | C57BL/6NTac | |
| 13382A-A5 | VGB6 | C57BL/6NTac | |
| 13382A-A9 | VGB6 | C57BL/6NTac | |
| 13382A-C11 | VGB6 | C57BL/6NTac | |
| 13382A-G7 | VGB6 | C57BL/6NTac | |
| 13382A-B10 | VGB6 | C57BL/6NTac | |
