| Gene | Avpr1b |
| Chromosome | 1 |
| Deletion Start | 131,599,744 |
| Deletion End | 131,609,827 |
| Deletion Size | 10,084 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,600,527 | 131,600,549 | GTCAGCAGCATCAGTACCATCTC |
| TUP | 131,600,550 | 131,600,575 | CAGGGCAAAGATCCGAACCGTGAAGA |
| TUR | 131,600,580 | 131,600,601 | GTAGGCCAGCACAATGACAAAG |
| TDF | 131,609,616 | 131,609,637 | CACCTGTGGTCCATCTACTCTC |
| TDP | 131,609,638 | 131,609,663 | CGTCTTAGCCTTAACCTCAGCCTCCA |
| TDR | 131,609,691 | 131,609,711 | TCCACCTGCTCCAAATCCTTC |
PCR Primers
| Primer | Sequence |
| SU | ACTTCCAACCTCCTCCAACC |
| SD | CCAGTCAGGTTAGAATCGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13386B-A1 | VGB6 | C57BL/6NTac | |
| 13386B-H9 | VGB6 | C57BL/6NTac | |
| 13386B-G5 | VGB6 | C57BL/6NTac | |
