| Gene | Gpank1 |
| Chromosome | 17 |
| Deletion Start | 35,122,989 |
| Deletion End | 35,124,717 |
| Deletion Size | 1,729 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,123,638 | 35,123,657 | GGAGCCCTGAGAACCAGAAC |
| TUP | 35,123,658 | 35,123,683 | CGGTGAGGAGGGAGCCTTCACTTACA |
| TUR | 35,123,698 | 35,123,718 | GGTGCCCAGGAAAGGAAGAAC |
| TDF | 35,124,586 | 35,124,608 | GAGGGAGAACAGAAGGCAAGAAG |
| TDP | 35,124,616 | 35,124,641 | CAGAGCCTGGGAGCGAGATCTAAGAC |
| TDR | 35,124,665 | 35,124,685 | ACCAGGGCCAGTCCATATCAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTGTATGTGTTAGCGGAATG |
| SD | CTCTAACCAAGCTATGCCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13388A-B2 | VGB6 | C57BL/6NTac | |
| 13388A-C1 | VGB6 | C57BL/6NTac | |
| 13388A-D7 | VGB6 | C57BL/6NTac | |
| 13388B-B6 | VGB6 | C57BL/6NTac | |
| 13388B-D4 | VGB6 | C57BL/6NTac | |
| 13388B-F9 | VGB6 | C57BL/6NTac | |
