13390   Bcl11a     Status: ES cell colonies screened / QC positives

GeneBcl11a
Chromosome11
Deletion Start24,078,394
Deletion End24,085,643
Deletion Size7,250
Genome Build38
MGI IDMGI:106190
NCBI Gene ID14025
BAC ID202K13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,078,39824,078,415GCCGCAAGCAAGGCAAAC
TUP24,078,41824,078,445CAGCACTTAAGCAAACGGGAATTCTCGC
TUR24,078,51624,078,540ATGCACACACCCCTCTCTTAACCTC
TDF24,084,69324,084,711GGCAGGGAGATGTTAGCAC
TDP24,084,71424,084,739TGACGCTCACCTCATTGTGATGGGTA
TDR24,084,74024,084,758CCACATTTGCACCGCAGTC

PCR Primers

PrimerSequence
SUTCTCCCACAATTCATCTTCC
SDTGGTGTGGTAAGGTTCAACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR143 bp++-
TDFTDR66 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13390A-E3VGB6C57BL/6NTac
13390A-D8VGB6C57BL/6NTac
13390C-D4VGB6C57BL/6NTac
13390C-D8VGB6C57BL/6NTac
13390C-G9VGB6C57BL/6NTac
13390C-E12VGB6C57BL/6NTac
13390C-F7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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