| Gene | Birc2 |
| Chromosome | 9 |
| Deletion Start | 7,834,475 |
| Deletion End | 7,818,682 |
| Deletion Size | 15,794 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,834,060 | 7,834,081 | GTAGATTTGCACATTCGTCACC |
| TUP | 7,834,035 | 7,834,059 | TCTGGAACGAGGTGGCATTCACTCC |
| TUR | 7,834,008 | 7,834,031 | AGAATTAAGAGGGCTAGAGCACAG |
| TDF | 7,818,924 | 7,818,944 | GGCTTGTCATTGGAAGAGCAG |
| TDP | 7,818,893 | 7,818,922 | TGCGGAGATTACAAGAAGAACGAACTTGCA |
| TDR | 7,818,820 | 7,818,839 | GCACATTCCTGGCAGACTAC |
PCR Primers
| Primer | Sequence |
| SU | GTACCTAGGCTTCATAACTAG |
| SD | GCATACATCCCTGCACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13394A-C5 | VGB6 | C57BL/6NTac | |
| 13394A-F6 | VGB6 | C57BL/6NTac | |
| 13394A-H3 | VGB6 | C57BL/6NTac | |
