| Gene | Brms1 |
| Chromosome | 19 |
| Deletion Start | 5,045,903 |
| Deletion End | 5,049,507 |
| Deletion Size | 3,605 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,046,657 | 5,046,678 | CAAGGGCTTCTGTCTGGATGTG |
| TUP | 5,046,687 | 5,046,715 | TAAGTATGAGTGTGAACTCCAGGGAGCCA |
| TUR | 5,046,769 | 5,046,788 | GGACCTGCCATACCTCAGTC |
| TDF | 5,049,169 | 5,049,191 | CTGGAGATGTAGCTCAGTGGTAG |
| TDP | 5,049,192 | 5,049,211 | TGCGCTTGCCGAACATGGGC |
| TDR | 5,049,214 | 5,049,231 | GGGATTGGGACTGGGTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGTAAAGGAGCAGGGCTTC |
| SD | GGAATAGCCCTGAGAAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13396A-H3 | VGB6 | C57BL/6NTac | |
| 13396A-E2 | VGB6 | C57BL/6NTac | |
| 13396A-F8 | VGB6 | C57BL/6NTac | |
| 13396A-G7 | VGB6 | C57BL/6NTac | |
| 13396A-F5 | VGB6 | C57BL/6NTac | |
