| Gene | Fam203a |
| Chromosome | 15 |
| Deletion Start | 76,368,959 |
| Deletion End | 76,370,746 |
| Deletion Size | 1,788 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,369,611 | 76,369,631 | GGAAGTGAACAAGGACAGCAG |
| TUP | 76,369,638 | 76,369,663 | TCTTCTTTCAACAGGTGCGTGGTCCA |
| TUR | 76,369,683 | 76,369,705 | CGCACCGAAGAATCTGTGTACTG |
| TDF | 76,370,108 | 76,370,126 | GCAGGGCTTGGGTGTTCAG |
| TDP | 76,370,127 | 76,370,151 | CACAGGGACCAATAGTCTCCCACCC |
| TDR | 76,370,192 | 76,370,210 | GGCTCCCGTTGCTTGTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCGCTTCAAATTATGCGTG |
| SD | GGATGGCAGAGCATTCTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13397B-C3 | VGB6 | C57BL/6NTac | |
| 13397B-H5 | VGB6 | C57BL/6NTac | |
