| Gene | Bsg |
| Chromosome | 10 |
| Deletion Start | 79,704,521 |
| Deletion End | 79,711,682 |
| Deletion Size | 7,162 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,705,099 | 79,705,119 | TGACGCTGGAAGACAGTAAGG |
| TUP | 79,705,128 | 79,705,154 | CAACATCTCCAACGTGAGTGAGGGCGT |
| TUR | 79,705,188 | 79,705,205 | TGGTACGCAGGTGGAGAC |
| TDF | 79,711,123 | 79,711,142 | GTTGGCCTCACCATGTGTTG |
| TDP | 79,711,145 | 79,711,173 | CATCTGATGTACGACACTTCTGCAGCACC |
| TDR | 79,711,195 | 79,711,214 | TCAGGGCTCGTGTGAACTTG |
PCR Primers
| Primer | Sequence |
| SU | TCAAGGGCAGTCCAGGCTC |
| SD | GACCTCAACACTCATTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13398A-B2 | VGB6 | C57BL/6NTac | |
| 13398A-B12 | VGB6 | C57BL/6NTac | |
| 13398A-E2 | VGB6 | C57BL/6NTac | |
| 13398A-F3 | VGB6 | C57BL/6NTac | |
