| Gene | Tprn |
| Chromosome | 2 |
| Deletion Start | 25,263,465 |
| Deletion End | 25,269,426 |
| Deletion Size | 5,962 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,263,679 | 25,263,699 | CCAGCGCCAATGATTCCTTTG |
| TUP | 25,263,704 | 25,263,726 | AAGGCCATCCTCCAAGCCAGACA |
| TUR | 25,263,780 | 25,263,799 | AAGGAGTTGCGGGAGTTCAC |
| TDF | 25,268,605 | 25,268,622 | TAGCACCAACAGCCCAAC |
| TDP | 25,268,625 | 25,268,651 | ATGGGTGTTAGCCTCTTAAGCGGGCCT |
| TDR | 25,268,658 | 25,268,679 | GAATCCTAGCACCAACCATCAC |
PCR Primers
| Primer | Sequence |
| SU | CGCTGCCGAGGTAGTAGTG |
| SD | GAGCTGAAATTTCCGACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13400A-B10 | VGB6 | C57BL/6NTac | |
| 13400A-E3 | VGB6 | C57BL/6NTac | |
| 13400A-E12 | VGB6 | C57BL/6NTac | |
| 13400A-G5 | VGB6 | C57BL/6NTac | |
