| Gene | Cabp4 |
| Chromosome | 19 |
| Deletion Start | 4,139,553 |
| Deletion End | 4,135,981 |
| Deletion Size | 3,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,139,502 | 4,139,523 | GGCTCCCAGAAGATCCCTAAAG |
| TUP | 4,139,474 | 4,139,497 | TGTATCTCCCAGGAGTGCTGCTGA |
| TUR | 4,139,444 | 4,139,464 | TTGCTCCTTCTCCTGGTCAAG |
| TDF | 4,136,138 | 4,136,158 | TGCGAGAAATGGACCTCAATG |
| TDP | 4,136,106 | 4,136,134 | ATGGCACCATAGACTTTGACGGTGAGTCA |
| TDR | 4,136,074 | 4,136,093 | GTGCTAGAGGCATGGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | TATGCCTCCTCCTGCCTAAG |
| SD | CAGTGAAAACTGTAACCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13402A-H3 | VGB6 | C57BL/6NTac | |
| 13402A-H6 | VGB6 | C57BL/6NTac | |
| 13402A-H7 | VGB6 | C57BL/6NTac | |
| 13402A-F8 | VGB6 | C57BL/6NTac | |
| 13402A-G8 | VGB6 | C57BL/6NTac | |
