| Gene | Calca |
| Chromosome | 7 |
| Deletion Start | 114,635,209 |
| Deletion End | 114,632,428 |
| Deletion Size | 2,782 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,634,676 | 114,634,698 | CCTAAACTTATGAGGACGCTGAC |
| TUP | 114,634,650 | 114,634,674 | CAAGCCTCTAGTGTAAGCGGCAGGC |
| TUR | 114,634,590 | 114,634,611 | GCTGCTTTCCAAGATTGACCTG |
| TDF | 114,633,173 | 114,633,194 | GTCGTAGTTTGGTGAGCGATGG |
| TDP | 114,633,138 | 114,633,163 | AGCTCAAGCAGTAAGAAGAGCGGCGA |
| TDR | 114,633,115 | 114,633,137 | CAGCAGTGTTTACCTGTTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGACAGGAGACACCTGGG |
| SD | GGCTTTCAAACCCATATGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13404A-F3 | VGB6 | C57BL/6NTac | |
| 13404A-D10 | VGB6 | C57BL/6NTac | |
| 13404A-D12 | VGB6 | C57BL/6NTac | |
| 13404A-E5 | VGB6 | C57BL/6NTac | |
| 13404A-A6 | VGB6 | C57BL/6NTac | |
