| Gene | Cby1 |
| Chromosome | 15 |
| Deletion Start | 79,661,450 |
| Deletion End | 79,667,171 |
| Deletion Size | 5,722 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,661,977 | 79,661,995 | GAGGACCTGGGTTGGGTTC |
| TUP | 79,661,998 | 79,662,020 | AGCCCGGACAAGGATGGCTCACA |
| TUR | 79,662,094 | 79,662,116 | GCTCTGCCATCATGTATGTTGTG |
| TDF | 79,666,435 | 79,666,454 | GCTGGTGGGCAGCTTCTTTG |
| TDP | 79,666,456 | 79,666,477 | CCCACCGGCTACCCACTCTGTG |
| TDR | 79,666,479 | 79,666,499 | TGTCTTGCTGGAGTGATGAGG |
PCR Primers
| Primer | Sequence |
| SU | TTCCAGAACTATTAGGACCTC |
| SD | AGGCAGAGAGATTACGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 140 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13406A-H1 | VGB6 | C57BL/6NTac | |
| 13406A-G1 | VGB6 | C57BL/6NTac | |
| 13406A-D9 | VGB6 | C57BL/6NTac | |
| 13406A-E1 | VGB6 | C57BL/6NTac | |
| 13406A-H7 | VGB6 | C57BL/6NTac | |
| 13406A-D3 | VGB6 | C57BL/6NTac | |
