| Gene | Ccdc33 |
| Chromosome | 9 |
| Deletion Start | 58,101,939 |
| Deletion End | 58,076,542 |
| Deletion Size | 25,398 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,101,327 | 58,101,345 | CTGCGTCAGGCACTGTAAC |
| TUP | 58,101,300 | 58,101,325 | TCTGCCATTATCGTAAATCCCGAGGC |
| TUR | 58,101,248 | 58,101,268 | TTCTTCACCTGGGCTCATTTC |
| TDF | 58,077,158 | 58,077,175 | GCAGCTCGTTCGTTGTCC |
| TDP | 58,077,126 | 58,077,150 | CAGATGACATGAGGCCTGCTGCAGT |
| TDR | 58,077,100 | 58,077,122 | CAGTTTGGGCCTATTGGTGTATG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTGCTTAGCTGTCCATG |
| SD | ATGACTGCTGACTTGAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13410A-A5 | VGB6 | C57BL/6NTac | |
| 13410A-A6 | VGB6 | C57BL/6NTac | |
| 13410A-G12 | VGB6 | C57BL/6NTac | |
| 13410A-A7 | VGB6 | C57BL/6NTac | |
| 13410A-B6 | VGB6 | C57BL/6NTac | |
