| Gene | Ccdc53 |
| Chromosome | 10 |
| Deletion Start | 88,201,245 |
| Deletion End | 88,219,355 |
| Deletion Size | 18,111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,201,835 | 88,201,856 | AACCGTGGCCTTTCTAAACCAG |
| TUP | 88,201,861 | 88,201,884 | TGGTGCACACTGTGCAGTTTCTCA |
| TUR | 88,201,899 | 88,201,919 | GGGACCTACCTCCTCACAAAC |
| TDF | 88,219,252 | 88,219,274 | GAATAGCACGCAAGACTCTGGAG |
| TDP | 88,219,275 | 88,219,299 | CACAGGAGAGTGAAGCGCCATCAGA |
| TDR | 88,219,304 | 88,219,322 | CCTTGGCTACGGTCAGGAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCCAGTCTGACCTCATCC |
| SD | GCATATGGAAAGCAAACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13411A-B1 | VGB6 | C57BL/6NTac | |
| 13411A-G3 | VGB6 | C57BL/6NTac | |
| 13411A-B12 | VGB6 | C57BL/6NTac | |
