| Gene | Ccdc64 |
| Chromosome | 5 |
| Deletion Start | 115,731,556 |
| Deletion End | 115,724,545 |
| Deletion Size | 7,012 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,731,122 | 115,731,142 | GAGCTGACGGACAAGCTAGAG |
| TUP | 115,731,101 | 115,731,120 | TGAGGACCTTCCGGCGGCAT |
| TUR | 115,731,045 | 115,731,066 | GAGGTAGACTAGGCACGGAAAC |
| TDF | 115,724,738 | 115,724,761 | CCCAGCACCTAGAACAAGAGAAAC |
| TDP | 115,724,709 | 115,724,736 | TGAACTGAGAAGGCGATTCGAGAATCGA |
| TDR | 115,724,632 | 115,724,651 | TGCTGCCTCTCCAACTCATC |
PCR Primers
| Primer | Sequence |
| SU | ATCCTTAGCGCGGCTTCTC |
| SD | TACAAGTGACGACGGACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13413A-A10 | VGB6 | C57BL/6NTac | |
| 13413A-B11 | VGB6 | C57BL/6NTac | |
| 13413A-G11 | VGB6 | C57BL/6NTac | |
| 13413A-E6 | VGB6 | C57BL/6NTac | |
