| Gene | Ccno |
| Chromosome | 13 |
| Deletion Start | 112,987,901 |
| Deletion End | 112,990,085 |
| Deletion Size | 2,185 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,988,645 | 112,988,665 | GACTTGCTGAGAGGGTTAGAC |
| TUP | 112,988,666 | 112,988,690 | TGGGTAGTGTGCCCTAATGCACTGT |
| TUR | 112,988,733 | 112,988,752 | CTGCCTTAAACCAGGCGTTC |
| TDF | 112,989,932 | 112,989,949 | GAGGCCACACTGCAAGAC |
| TDP | 112,989,952 | 112,989,972 | TCTGGGCAAGCTGCAGACGCT |
| TDR | 112,989,979 | 112,989,999 | GGCAGGGAGCTGCTATTGATG |
PCR Primers
| Primer | Sequence |
| SU | GAGTTGCAACGCCTCCTC |
| SD | GGAACTCAGCCTCCTGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13416A-B10 | VGB6 | C57BL/6NTac | |
| 13416A-C4 | VGB6 | C57BL/6NTac | |
| 13416A-C10 | VGB6 | C57BL/6NTac | |
| 13416A-D7 | VGB6 | C57BL/6NTac | |
| 13416A-D9 | VGB6 | C57BL/6NTac | |
| 13416A-E3 | VGB6 | C57BL/6NTac | |
