| Gene | Cdadc1 |
| Chromosome | 14 |
| Deletion Start | 59,597,833 |
| Deletion End | 59,564,498 |
| Deletion Size | 33,336 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,597,449 | 59,597,469 | TGTGCTGTCCAGGCTTCATAG |
| TUP | 59,597,426 | 59,597,446 | CGCGTCCTGTCCTGCACTGGT |
| TUR | 59,597,373 | 59,597,393 | GCGCAATTTCAGGCTCTTCAG |
| TDF | 59,564,992 | 59,565,012 | CCCTGACACGTCTCTTCTGAG |
| TDP | 59,564,964 | 59,564,989 | TGCCAGCTTCTTATCTTGGGAGCCTG |
| TDR | 59,564,936 | 59,564,956 | GCCTAGAGCAGCGAGAATAGC |
PCR Primers
| Primer | Sequence |
| SU | CGGTTCTATGTTCGCCTCTC |
| SD | CCTGGCTGGTAGAAGGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13421A-C3 | VGB6 | C57BL/6NTac | |
| 13421A-G7 | VGB6 | C57BL/6NTac | |
| 13421A-F3 | VGB6 | C57BL/6NTac | |
| 13421A-C4 | VGB6 | C57BL/6NTac | |
| 13421A-H7 | VGB6 | C57BL/6NTac | |
| 13421A-E6 | VGB6 | C57BL/6NTac | |
