13422 Cdkn1b Status: ES cell colonies screened / QC no positives
| Gene | Cdkn1b |
| Chromosome | 6 |
| Deletion Start | 134,920,923 |
| Deletion End | 134,922,168 |
| Deletion Size | 1,246 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,921,014 | 134,921,031 | TCTTCGGCCCGGTCAATC |
| TUP | 134,921,035 | 134,921,062 | AAGAACTAACCCGGGACTTGGAGAAGCA |
| TUR | 134,921,076 | 134,921,093 | GCGCTGACTCGCTTCTTC |
| TDF | 134,922,022 | 134,922,040 | CTGGCACTGTGGAGCAGAC |
| TDP | 134,922,042 | 134,922,062 | CCCAAGAAGCCCGGCCTTCGA |
| TDR | 134,922,071 | 134,922,090 | AACCCACCGGAGCTGTTTAC |
PCR Primers
| Primer | Sequence |
| SU | CCTACGCTCCGACTGTTTG |
| SD | AACCTCTGGGAAATGGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
