| Gene | Cep97 |
| Chromosome | 16 |
| Deletion Start | 55,934,792 |
| Deletion End | 55,905,069 |
| Deletion Size | 29,724 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,934,268 | 55,934,288 | ACCAGTAGCCGTGGTAGAATC |
| TUP | 55,934,240 | 55,934,265 | AGAATGACCCAGACACGCGTTGCTCC |
| TUR | 55,934,181 | 55,934,199 | TGGGCTCTGCCAAACAAAG |
| TDF | 55,905,346 | 55,905,365 | CGTCACCTCGCTTGTTCTCC |
| TDP | 55,905,316 | 55,905,342 | AAGGTTTGACGCCTCCTCTGACAGTGA |
| TDR | 55,905,266 | 55,905,287 | CTGGCGTCTGGCTAATTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TCGAATTTCTCAAACACCTGG |
| SD | GGCTTTCCTTAACACAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13424A-B2 | VGB6 | C57BL/6NTac | |
| 13424B-H1 | VGB6 | C57BL/6NTac | |
