| Gene | Cfb |
| Chromosome | 17 |
| Deletion Start | 34,862,103 |
| Deletion End | 34,856,333 |
| Deletion Size | 5,771 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,861,286 | 34,861,308 | GGACTAGGCAGGTACTGTTTCTC |
| TUP | 34,861,255 | 34,861,283 | TCACAGGATTTCTAAGACCAGGAAGGACC |
| TUR | 34,861,199 | 34,861,219 | GTGGGCAGCGTATTGCTTTAG |
| TDF | 34,856,526 | 34,856,544 | AGACCAGAGGCGGCAACAG |
| TDP | 34,856,502 | 34,856,525 | CTGGTACCCTCTTATGCCCGGGAC |
| TDR | 34,856,471 | 34,856,489 | AGGGCAGCACCTGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGGGAGCTTTGAAAGGG |
| SD | AGTGAGGTTATGGCCACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13425A-H5 | VGB6 | C57BL/6NTac | |
| 13425A-A2 | VGB6 | C57BL/6NTac | |
