| Gene | Chchd1 |
| Chromosome | 14 |
| Deletion Start | 20,703,077 |
| Deletion End | 20,704,307 |
| Deletion Size | 1,231 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,703,398 | 20,703,416 | CCAGGGCTCAGGTGATGTC |
| TUP | 20,703,417 | 20,703,439 | TGGCTCCTAGCGTGCCTTCTTGG |
| TUR | 20,703,457 | 20,703,478 | CCCGGCTTGAACCATTTCTTTG |
| TDF | 20,704,116 | 20,704,137 | TTCAGGAAGCCAGGAAGATGAG |
| TDP | 20,704,144 | 20,704,168 | CCAGGAGTCTCTGGGACAGTCCGAG |
| TDR | 20,704,189 | 20,704,213 | GGGAAATCTCTGTAACAACTTGGTC |
PCR Primers
| Primer | Sequence |
| SU | CACAAATAAGTGGCAGCCG |
| SD | GGAAATGAAGAGCATCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13426A-C2 | VGB6 | C57BL/6NTac | |
| 13426A-E6 | VGB6 | C57BL/6NTac | |
| 13426A-D1 | VGB6 | C57BL/6NTac | |
| 13426A-D3 | VGB6 | C57BL/6NTac | |
| 13426A-G1 | VGB6 | C57BL/6NTac | |
| 13426A-E2 | VGB6 | C57BL/6NTac | |
