13427   Chrm4     Status: ES cell colonies screened / QC positives

GeneChrm4
Chromosome2
Deletion Start91,927,252
Deletion End91,928,781
Deletion Size1,530
Genome Build38
MGI IDMGI:88399
NCBI Gene ID12672
BAC ID309I13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF91,927,27491,927,292GCAGCTCAGCCAATCAGTC
TUP91,927,29391,927,313TGTGCGCCTGGTCACAACAGC
TUR91,927,33691,927,356TGTCGCAATGAACACCATCTC
TDF91,928,46591,928,483TGCTGGCCTTCATCCTCAC
TDP91,928,48591,928,511TGGACACCCTACAATGTCATGGTCCTG
TDR91,928,54291,928,561CCGATGGACCACACCCTTTC

PCR Primers

PrimerSequence
SUGCAAGCACCAAGTTCTCTCC
SDTACATGCAAGGGAGAGGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR83 bp++-
TDFTDR97 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13427A-H11VGB6C57BL/6NTac
13427A-A3VGB6C57BL/6NTac
13427A-B3VGB6C57BL/6NTac
13427A-F3VGB6C57BL/6NTac
13427A-F8VGB6C57BL/6NTac
13427A-H3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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