13428 Ckb Status: ES cell colonies screened / QC no positives
| Gene | Ckb |
| Chromosome | 12 |
| Deletion Start | 111,671,955 |
| Deletion End | 111,669,502 |
| Deletion Size | 2,454 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,671,050 | 111,671,071 | CCTGTCCTTGAACTCTGCACTC |
| TUP | 111,671,023 | 111,671,047 | CAGCTCTGTCCAGCCTAGATGGCGA |
| TUR | 111,670,979 | 111,670,998 | CCGCCTCAGTCATGCTCTTG |
| TDF | 111,670,436 | 111,670,456 | AGCTGCCTTCCTCCACTGTTG |
| TDP | 111,670,406 | 111,670,429 | TTGGCGTCTTGGTGACAGCTCACA |
| TDR | 111,670,309 | 111,670,327 | TGGAGTCCCTGCCAGAATG |
PCR Primers
| Primer | Sequence |
| SU | TGCAAGCACAGGCATCCATCG |
| SD | AGATGACACAGAAGGCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
