| Gene | Cldn13 |
| Chromosome | 5 |
| Deletion Start | 134,915,326 |
| Deletion End | 134,914,478 |
| Deletion Size | 849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,915,261 | 134,915,278 | TGGGTGGGTGCCATTGTG |
| TUP | 134,915,239 | 134,915,258 | CTGCGTGCTGCCCGTTTGGA |
| TUR | 134,915,208 | 134,915,231 | GGGTCTGTCTCATCATCTGGAAAG |
| TDF | 134,914,786 | 134,914,804 | GGAGGCATCCTGCTCTGTG |
| TDP | 134,914,757 | 134,914,785 | TCAACATCCCAGTATGCAGAGACTTTCCC |
| TDR | 134,914,712 | 134,914,731 | GTTGTTGGCTCCTGAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CCCAGAGATCTCGTCCAGAG |
| SD | GACCTTCAGGAAAGTGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13429A-A5 | VGB6 | C57BL/6NTac | |
| 13429A-B1 | VGB6 | C57BL/6NTac | |
| 13429A-B3 | VGB6 | C57BL/6NTac | |
| 13429A-H3 | VGB6 | C57BL/6NTac | |
| 13429A-D5 | VGB6 | C57BL/6NTac | |
| 13429A-D11 | VGB6 | C57BL/6NTac | |
