| Gene | Cox6b2 |
| Chromosome | 7 |
| Deletion Start | 4,752,832 |
| Deletion End | 4,751,840 |
| Deletion Size | 993 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,752,518 | 4,752,540 | CCGCGTTGGAGTGAAATGTATTG |
| TUP | 4,752,493 | 4,752,517 | AGCCAGAAGGGATGGAGCCTTGTCT |
| TUR | 4,752,472 | 4,752,492 | TGTCCTGGTCTTGCCCTTTAC |
| TDF | 4,752,011 | 4,752,031 | GGGTAGTGGCTTCAGACTCAG |
| TDP | 4,751,972 | 4,751,992 | TCCCTCTGCCACTGCAGGTGC |
| TDR | 4,751,943 | 4,751,964 | TCCCTGCTTGATCTGCTCATTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCCTACAAGAACCAAGGG |
| SD | GGCTGCCTAACTCACAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13439A-B5 | VGB6 | C57BL/6NTac | |
| 13439A-B7 | VGB6 | C57BL/6NTac | |
| 13439A-C2 | VGB6 | C57BL/6NTac | |
| 13439A-D10 | VGB6 | C57BL/6NTac | |
| 13439A-H5 | VGB6 | C57BL/6NTac | |
