| Gene | Creb3l4 |
| Chromosome | 3 |
| Deletion Start | 90,242,829 |
| Deletion End | 90,237,720 |
| Deletion Size | 5,110 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,241,982 | 90,242,001 | GCCCTCCGATTCGCATAGAC |
| TUP | 90,241,958 | 90,241,979 | ATCCTGCCTAGGGTCAGCACCA |
| TUR | 90,241,912 | 90,241,931 | TGGTTGGCGTCGAGACTTAC |
| TDF | 90,238,564 | 90,238,583 | CCAAGCCTGCTCTGCTTCTC |
| TDP | 90,238,536 | 90,238,560 | ACTCCCTAGAGTGGCGGCCTGTTCG |
| TDR | 90,238,499 | 90,238,520 | GCTCCTGGACCTTTCTCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTACTCCCAGATGCAGGTCC |
| SD | TGCCCATATGTTATGGATTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13443A-B3 | VGB6 | C57BL/6NTac | |
| 13443A-C5 | VGB6 | C57BL/6NTac | |
| 13443A-H10 | VGB6 | C57BL/6NTac | |
| 13443A-G10 | VGB6 | C57BL/6NTac | |
| 13443A-E12 | VGB6 | C57BL/6NTac | |
| 13443A-G11 | VGB6 | C57BL/6NTac | |
