| Gene | Cryaa |
| Chromosome | 17 |
| Deletion Start | 31,678,004 |
| Deletion End | 31,681,197 |
| Deletion Size | 3,194 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,678,140 | 31,678,159 | ACTACCGCCAGTCCCTCTTC |
| TUP | 31,678,162 | 31,678,185 | CACTGTGCTGGACTCGGGCATCTC |
| TUR | 31,678,204 | 31,678,224 | AGCAGCTAGGAGGAACCAGTG |
| TDF | 31,681,026 | 31,681,045 | ACCGTCTGCCTTCCAATGTG |
| TDP | 31,681,047 | 31,681,068 | ACCAGTCCGCCCTCTCCTGCTC |
| TDR | 31,681,112 | 31,681,131 | CCAGCATCCAAACCGGACTG |
PCR Primers
| Primer | Sequence |
| SU | CTTTGAACACAACGCTTTCG |
| SD | ATTCTCATGGATGACCAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13446A-F7 | VGB6 | C57BL/6NTac | |
| 13446A-D5 | VGB6 | C57BL/6NTac | |
| 13446A-E7 | VGB6 | C57BL/6NTac | |
| 13446A-B10 | VGB6 | C57BL/6NTac | |
| 13446A-H2 | VGB6 | C57BL/6NTac | |
