| Gene | Cryge |
| Chromosome | 1 |
| Deletion Start | 65,051,110 |
| Deletion End | 65,048,520 |
| Deletion Size | 2,591 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,050,444 | 65,050,465 | TGAGGTTGTTCACCCATGTTTC |
| TUP | 65,050,410 | 65,050,437 | TCATTCACATCAGACCTTGTCACCAGGG |
| TUR | 65,050,360 | 65,050,383 | CCATCCCTTGGCAAGTATTACCAC |
| TDF | 65,049,464 | 65,049,484 | GAGGTTGTAAAGGGCCGGAAG |
| TDP | 65,049,435 | 65,049,463 | CAACAAAGAACGCTGGAGGGAGGGATATC |
| TDR | 65,049,403 | 65,049,425 | GGATGGGCCAATTCAGGAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCATCATCCTGTGGAGAAGG |
| SD | AGATTCTCGACTGCACTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13447A-A5 | VGB6 | C57BL/6NTac | |
| 13447A-A6 | VGB6 | C57BL/6NTac | |
| 13447A-B6 | VGB6 | C57BL/6NTac | |
| 13447A-E3 | VGB6 | C57BL/6NTac | |
| 13447A-C11 | VGB6 | C57BL/6NTac | |
| 13447A-G8 | VGB6 | C57BL/6NTac | |
