| Gene | Csf3r |
| Chromosome | 4 |
| Deletion Start | 126,027,340 |
| Deletion End | 126,043,893 |
| Deletion Size | 16,554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,027,493 | 126,027,513 | AGCAGCCAAAGCCTCTCCATC |
| TUP | 126,027,515 | 126,027,541 | CAGAGCTTCGAGGTAGCAGAGCAGTTA |
| TUR | 126,027,582 | 126,027,600 | GCATCCCAGTGAACCAGAC |
| TDF | 126,043,197 | 126,043,218 | TGTCAGGGACATGGTAGGAATG |
| TDP | 126,043,220 | 126,043,243 | ACAGCTGCCAGGCAAGTATGTCCC |
| TDR | 126,043,258 | 126,043,278 | GGCATCTCCCTTGTTGCTATG |
PCR Primers
| Primer | Sequence |
| SU | AGCTCCATCCTATCCCTGC |
| SD | CCTACAATCAGTTCAATGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13448A-B8 | VGB6 | C57BL/6NTac | |
| 13448A-H10 | VGB6 | C57BL/6NTac | |
| 13448A-E2 | VGB6 | C57BL/6NTac | |
