| Gene | Cst3 |
| Chromosome | 2 |
| Deletion Start | 148,875,374 |
| Deletion End | 148,871,859 |
| Deletion Size | 3,516 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,874,562 | 148,874,583 | CGAACACTCAGGTCCTTCACTC |
| TUP | 148,874,530 | 148,874,557 | TCTCAGTTTGCTGGTCTCCACTATGGAC |
| TUR | 148,874,492 | 148,874,514 | GGCTGCCAGACTGGATAGAAATG |
| TDF | 148,872,806 | 148,872,829 | CCATCTGATGAGGGTAGGTACTTG |
| TDP | 148,872,778 | 148,872,805 | ATATCAGCCTAAGGGCAATCGTGCAGGC |
| TDR | 148,872,731 | 148,872,750 | TGTGCCCAACACCTGTATCC |
PCR Primers
| Primer | Sequence |
| SU | AGGGCACAGAAGAGGAACTG |
| SD | CCCATAGACACCAGTGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13449A-A2 | VGB6 | C57BL/6NTac | |
| 13449A-A9 | VGB6 | C57BL/6NTac | |
| 13449A-A10 | VGB6 | C57BL/6NTac | |
| 13449A-B4 | VGB6 | C57BL/6NTac | |
| 13449A-F2 | VGB6 | C57BL/6NTac | |
| 13449A-H7 | VGB6 | C57BL/6NTac | |
