| Gene | Cst6 |
| Chromosome | 19 |
| Deletion Start | 5,349,331 |
| Deletion End | 5,347,874 |
| Deletion Size | 1,458 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,348,767 | 5,348,784 | TGCCAGGTGGAGTGGAAG |
| TUP | 5,348,738 | 5,348,764 | CCCAGTGTAGGTGCCACATACCATTCA |
| TUR | 5,348,715 | 5,348,736 | TGTCTGCGGCTAGACTGTAAAG |
| TDF | 5,348,337 | 5,348,358 | TGAGGCCCTTAGCTTGTCTATC |
| TDP | 5,348,307 | 5,348,332 | CCAGTCTATCTGGTCAGGCAGAAGCC |
| TDR | 5,348,260 | 5,348,279 | CACCCTGGCCTGAGTCATAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGGCAACTCAAACGTCAG |
| SD | CTGGACTGGCTCACACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13450A-A8 | VGB6 | C57BL/6NTac | |
| 13450A-B5 | VGB6 | C57BL/6NTac | |
| 13450A-D4 | VGB6 | C57BL/6NTac | |
| 13450A-G3 | VGB6 | C57BL/6NTac | |
| 13450A-G2 | VGB6 | C57BL/6NTac | |
| 13450A-E2 | VGB6 | C57BL/6NTac | |
