| Gene | Ctsf |
| Chromosome | 19 |
| Deletion Start | 4,855,271 |
| Deletion End | 4,860,530 |
| Deletion Size | 5,260 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,855,717 | 4,855,734 | TCTCCCTGGAAGCCACAC |
| TUP | 4,855,736 | 4,855,760 | AGAGGAGCCACCTTGCAATGATCCC |
| TUR | 4,855,792 | 4,855,812 | TGACCTCTTCACTCACCACTG |
| TDF | 4,860,449 | 4,860,468 | CAGCCGTGGTGAACTGAGAG |
| TDP | 4,860,470 | 4,860,494 | TGCTGGCTCAGGACCTATGTTGTCA |
| TDR | 4,860,508 | 4,860,526 | GGGCATGAGTCGGGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | ACTGACCTCACAGGTCTGCC |
| SD | TAGAGAAGGCCGGTCACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13452A-H7 | VGB6 | C57BL/6NTac | |
| 13452A-B11 | VGB6 | C57BL/6NTac | |
| 13452A-C10 | VGB6 | C57BL/6NTac | |
| 13452A-D11 | VGB6 | C57BL/6NTac | |
| 13452A-G9 | VGB6 | C57BL/6NTac | |
| 13452A-E11 | VGB6 | C57BL/6NTac | |
