| Gene | Cyc1 |
| Chromosome | 15 |
| Deletion Start | 76,343,567 |
| Deletion End | 76,345,789 |
| Deletion Size | 2,223 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,344,047 | 76,344,068 | CTCTGGGTCACTTAGGCTTGTC |
| TUP | 76,344,069 | 76,344,094 | TCCTGGGTTAAGTTCCACGAGCATTC |
| TUR | 76,344,097 | 76,344,115 | CGGCTCTGGAGGGCAAATG |
| TDF | 76,345,508 | 76,345,527 | GGATGTCGCCACCTTCCTTC |
| TDP | 76,345,530 | 76,345,554 | TGGGCATCAGAACCAGAGCATGACC |
| TDR | 76,345,572 | 76,345,591 | GCCCAACCGTTTACCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTGTTAATCCCACACAGG |
| SD | CATGCAAACAGTGCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13456A-A3 | VGB6 | C57BL/6NTac | |
| 13456A-B9 | VGB6 | C57BL/6NTac | |
| 13456A-H6 | VGB6 | C57BL/6NTac | |
| 13456A-D9 | VGB6 | C57BL/6NTac | |
| 13456A-F9 | VGB6 | C57BL/6NTac | |
| 13456A-E8 | VGB6 | C57BL/6NTac | |
