| Gene | D030056L22Rik |
| Chromosome | 19 |
| Deletion Start | 18,713,300 |
| Deletion End | 18,717,371 |
| Deletion Size | 4,072 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,713,889 | 18,713,909 | ACTGTCCTCTCCGCTGCTTTG |
| TUP | 18,713,913 | 18,713,937 | TGGTAGCCGCTAGCCATGTGATCAG |
| TUR | 18,713,986 | 18,714,007 | TTGGCGCTCCTACTCTTCTTAC |
| TDF | 18,716,917 | 18,716,938 | CTCCTTAAAGTCGGCCTCTGTC |
| TDP | 18,716,940 | 18,716,965 | TAGTGCGTGCCACCGAAGTAAATCGG |
| TDR | 18,716,994 | 18,717,013 | CCAGGCTGGGCTACCTACAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCTTCCATCCACATCCC |
| SD | TAAGCTTTGTGGTGCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13458D-C11 | VGB6 | C57BL/6NTac | |
| 13458D-D3 | VGB6 | C57BL/6NTac | |
| 13458D-E2 | VGB6 | C57BL/6NTac | |
| 13458D-F6 | VGB6 | C57BL/6NTac | |
| 13458D-G6 | VGB6 | C57BL/6NTac | |
| 13458E-E3 | VGB6 | C57BL/6NTac | |
| 13458E-F4 | VGB6 | C57BL/6NTac | |
| 13458E-G5 | VGB6 | C57BL/6NTac | |
| 13458E-H5 | VGB6 | C57BL/6NTac | |
| 13458E-H7 | VGB6 | C57BL/6NTac | |
