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Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,221,148 | 60,221,168 | CAGTACGAAGCCAGCAGTGTC |
| TUP | 60,221,126 | 60,221,147 | AGACTTCCCGACAGGCCAGCCA |
| TUR | 60,221,099 | 60,221,119 | ATCCGCATCAGCCTCTGTCTC |
| TDF | 60,200,382 | 60,200,404 | TTCCTGCTCAGCCTACAGAGTTG |
| TDP | 60,200,353 | 60,200,380 | TGAGGGAGCTTGAAGGGTCTCATGACTA |
| TDR | 60,200,304 | 60,200,327 | CCTGAACAGAAATCCTTCCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | CCTACTGACATTCTTGAGCC |
| SD | TAGACCAAACAGATCAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13460C-G1 | VGB6 | C57BL/6NTac |
