| Gene | Dnlz |
| Chromosome | 2 |
| Deletion Start | 26,352,009 |
| Deletion End | 26,350,367 |
| Deletion Size | 1,643 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,351,439 | 26,351,460 | GTCATCCAAGCGCATCTCAAAG |
| TUP | 26,351,416 | 26,351,438 | TTGGCCTATCACCAGGGCGTGGT |
| TUR | 26,351,392 | 26,351,409 | TGGCAACCAGGGCAAGTC |
| TDF | 26,350,679 | 26,350,701 | ACATTTGGTGAAGGTGCCATTAG |
| TDP | 26,350,655 | 26,350,677 | TGCCTGCAAGGTACTGGTGTCAC |
| TDR | 26,350,620 | 26,350,638 | CATCTTGGGCCTGGAACAC |
PCR Primers
| Primer | Sequence |
| SU | ATACGACAGGCAGCGACAAC |
| SD | GGGCTGGACACTAGCTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13461A-A1 | VGB6 | C57BL/6NTac | |
| 13461A-D6 | VGB6 | C57BL/6NTac | |
| 13461A-B2 | VGB6 | C57BL/6NTac | |
| 13461A-C9 | VGB6 | C57BL/6NTac | |
| 13461A-C3 | VGB6 | C57BL/6NTac | |
| 13461A-B9 | VGB6 | C57BL/6NTac | |
