| Gene | Fig4 |
| Chromosome | 10 |
| Deletion Start | 41,303,049 |
| Deletion End | 41,277,362 |
| Deletion Size | 25,688 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,302,814 | 41,302,835 | CACTGGATGGTTTGCAGGTCTG |
| TUP | 41,302,789 | 41,302,813 | TCTTGCTTAGGAACATGTGGCTGGC |
| TUR | 41,302,765 | 41,302,786 | ACCTGTCTGCACGCACTTCTTC |
| TDF | 41,277,726 | 41,277,744 | GGGACTGTGCCAGACTTTG |
| TDP | 41,277,700 | 41,277,725 | TGCATGCGAGGCAAGTACTCTACCAA |
| TDR | 41,277,680 | 41,277,699 | TGGCTGGAGATGTGGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | GCCTGCCCTTAAGAAAGATG |
| SD | CGCCATGTGAACCTTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13463A-H8 | VGB6 | C57BL/6NTac | |
| 13463A-B4 | VGB6 | C57BL/6NTac | |
| 13463A-F1 | VGB6 | C57BL/6NTac | |
| 13463A-E3 | VGB6 | C57BL/6NTac | |
| 13463A-D7 | VGB6 | C57BL/6NTac | |
| 13463A-C1 | VGB6 | C57BL/6NTac | |
