| Gene | Fdxacb1 |
| Chromosome | 9 |
| Deletion Start | 50,768,287 |
| Deletion End | 50,772,624 |
| Deletion Size | 4,338 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,769,079 | 50,769,100 | TGGCGGTCAGAGAACAACTTAC |
| TUP | 50,769,103 | 50,769,127 | AAGCGGGTCCTCCTTCCATCGTGTA |
| TUR | 50,769,142 | 50,769,163 | CCACCAAGCCTAGTAGCCAAAG |
| TDF | 50,771,972 | 50,771,992 | GGTCACCTGAAGGATGCTCTG |
| TDP | 50,771,996 | 50,772,023 | AACCTTCTGACTCAGACATTGCAGGAGG |
| TDR | 50,772,030 | 50,772,047 | CCACCGAAGTGCCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCAGTTACAGCGTCCTGAG |
| SD | CTAAGCATCGGGATCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13464A-B8 | VGB6 | C57BL/6NTac | |
| 13464A-D3 | VGB6 | C57BL/6NTac | |
| 13464A-H1 | VGB6 | C57BL/6NTac | |
| 13464A-H5 | VGB6 | C57BL/6NTac | |
| 13464A-H9 | VGB6 | C57BL/6NTac | |
