13465   Fitm2     Status: ES cell colonies screened / QC positives

GeneFitm2
Chromosome2
Deletion Start163,472,572
Deletion End163,469,491
Deletion Size3,082
Genome Build38
MGI IDMGI:2444508
NCBI Gene ID228859
BAC ID130O6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,472,263163,472,282GGACCCTGGGAAAGCAAGTG
TUP163,472,234163,472,258TCGCGCCTAGTATGTGAGCATTCCG
TUR163,472,211163,472,229CGTCACCGACTCGATCCTC
TDF163,469,815163,469,833GGGCCACTCCTTCCTCTTG
TDP163,469,789163,469,814ACCTTCTGTGCGCTCATGATTGTGGA
TDR163,469,770163,469,787ATGCAGCACGGCCATCTC

PCR Primers

PrimerSequence
SUAGAAAGCGAGAATCCCATCC
SDCCAGAGAGTGTCCTTGAGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR72 bp++-
TDFTDR64 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13465A-A2VGB6C57BL/6NTac
13465A-C8VGB6C57BL/6NTac
13465A-D3VGB6C57BL/6NTac
13465A-G11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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